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camp elisa detection kit  (R&D Systems)


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    R&D Systems camp elisa detection kit
    Camp Elisa Detection Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/camp elisa detection kit/product/R&D Systems
    Average 96 stars, based on 234 article reviews
    camp elisa detection kit - by Bioz Stars, 2026-06
    96/100 stars

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    The effects of GLP1R and PKA on the cAMP/PKA signaling pathway in EC cells. (A) The correlation between the expressions of GLP1R and PKA was analyzed by TIMER database ( http://cistrome.dfci.harvard.edu/TIMER/ ), rho = 0.181, p = 2.05e‐05. (B and C) The concentration of cAMP <t>in</t> <t>Ishikawa</t> and RL95‐2 cells was accessed by <t>ELISA.</t> (D–I) The detection of protein expressions (GLP1R, p‐PKA and PKA) in the Con, OE‐GLP1R, si‐PKA and si‐PKA + OE‐GLP1R groups was conducted by western blot, with GAPDH serving as the internal reference. All experiments were repeated three times to obtain average values. The data from three independent experiments were presented as the mean ± SD; * p < 0.05, ** p < 0.01; *** p < 0.001 versus Con; ^^ p < 0.01; ^^^ p < 0.001 versus OE‐GLP1R; ++ p < 0.01, +++ p < 0.001 versus si‐PKA. GLP1R, glucagon‐like peptide‐1 receptor; cAMP, cyclic adenosine monophosphate; Con, control; EC, endometrial carcinoma; ELISA, enzyme‐linked immunosorbent assay; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; OE‐GLP1R, GLP1R overexpression; PKA, protein kinase A; SD, standard deviation; si‐PKA, small interference RNA targeting PKA.
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    The effects of GLP1R and PKA on the cAMP/PKA signaling pathway in EC cells. (A) The correlation between the expressions of GLP1R and PKA was analyzed by TIMER database ( http://cistrome.dfci.harvard.edu/TIMER/ ), rho = 0.181, p = 2.05e‐05. (B and C) The concentration of cAMP in Ishikawa and RL95‐2 cells was accessed by ELISA. (D–I) The detection of protein expressions (GLP1R, p‐PKA and PKA) in the Con, OE‐GLP1R, si‐PKA and si‐PKA + OE‐GLP1R groups was conducted by western blot, with GAPDH serving as the internal reference. All experiments were repeated three times to obtain average values. The data from three independent experiments were presented as the mean ± SD; * p < 0.05, ** p < 0.01; *** p < 0.001 versus Con; ^^ p < 0.01; ^^^ p < 0.001 versus OE‐GLP1R; ++ p < 0.01, +++ p < 0.001 versus si‐PKA. GLP1R, glucagon‐like peptide‐1 receptor; cAMP, cyclic adenosine monophosphate; Con, control; EC, endometrial carcinoma; ELISA, enzyme‐linked immunosorbent assay; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; OE‐GLP1R, GLP1R overexpression; PKA, protein kinase A; SD, standard deviation; si‐PKA, small interference RNA targeting PKA.

    Journal: Journal of Clinical Laboratory Analysis

    Article Title: GLP1R inhibits the progression of endometrial carcinoma through activation of cAMP / PKA pathway

    doi: 10.1002/jcla.24604

    Figure Lengend Snippet: The effects of GLP1R and PKA on the cAMP/PKA signaling pathway in EC cells. (A) The correlation between the expressions of GLP1R and PKA was analyzed by TIMER database ( http://cistrome.dfci.harvard.edu/TIMER/ ), rho = 0.181, p = 2.05e‐05. (B and C) The concentration of cAMP in Ishikawa and RL95‐2 cells was accessed by ELISA. (D–I) The detection of protein expressions (GLP1R, p‐PKA and PKA) in the Con, OE‐GLP1R, si‐PKA and si‐PKA + OE‐GLP1R groups was conducted by western blot, with GAPDH serving as the internal reference. All experiments were repeated three times to obtain average values. The data from three independent experiments were presented as the mean ± SD; * p < 0.05, ** p < 0.01; *** p < 0.001 versus Con; ^^ p < 0.01; ^^^ p < 0.001 versus OE‐GLP1R; ++ p < 0.01, +++ p < 0.001 versus si‐PKA. GLP1R, glucagon‐like peptide‐1 receptor; cAMP, cyclic adenosine monophosphate; Con, control; EC, endometrial carcinoma; ELISA, enzyme‐linked immunosorbent assay; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; OE‐GLP1R, GLP1R overexpression; PKA, protein kinase A; SD, standard deviation; si‐PKA, small interference RNA targeting PKA.

    Article Snippet: The content of cAMP in Ishikawa and RL95‐2 cells was assessed using the cAMP ELISA Kit (ab133051, Abcam) according to the specification of manufacturer.

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Control, Over Expression, Standard Deviation